human normal liver cell line thle 3  (ATCC)

Journal: BMC Cancer

Article Title: Circ_0064288 acts as an oncogene of hepatocellular carcinoma cells by inhibiting miR-335-5p expression and promoting ROCK1 expression

doi: 10.1186/s12885-022-09323-8

Figure Lengend Snippet: Circ_0064288 expression is up-modulated in HCC. A - B . Volcano plots were utilized to show the differentially expressed circRNAs in HCC, with the data of GSE94508 and GSE97332. C . Venn diagram was employed to screen the circRNAs that were remarkably up-modulated in both datasets. D . Circ_0064288 expression in HCC tissues and paracancerous tissues was examined by qRT-PCR, and then the fold change of circ_0064288 expression in each HCC sample was shown. E . Kaplan-Meier curve was applied to analyze the relationship between circ_0064288 expression and the overall survival of HCC patients. F . Circ_0064288 expression in normal hepatic epithelial cell line (THLE-3) and HCC cell lines (Huh7, Hep3B, HCCLM3, and MHCC97-L) was measured by qRT-PCR. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Human HCC cell lines (Huh7, Hep3B, HCCLM3, and MHCC97-L) and human normal liver epithelial cell line (THLE-3) were procured from American Type Culture Collection (ATCC, Manassas, VA, USA) and Shanghai Cell Bank of Chinese Academy of Sciences (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR

Journal: BMC Cancer

Article Title: Circ_0064288 acts as an oncogene of hepatocellular carcinoma cells by inhibiting miR-335-5p expression and promoting ROCK1 expression

doi: 10.1186/s12885-022-09323-8

Figure Lengend Snippet: Circ_0064288 functions as ceRNA and sponges miR-335-5p. A . Bioinformatics databases (StarBase and Circular RNA Interactome) were used to predict the binding site of circ_0064288 to miR-335-5p. B . qRT-PCR was employed to detect circ_0064288 expression in the cytoplasm and nucleus of Huh7 and Hep3B cells. C . Dual-luciferase reporter gene experiment showed that the predicted binding sequence between circ_0064288 and miR-335-5p was functional. D . qRT-PCR was employed to detect miR-335-5p expression in HCC tissues and paracancerous tissues. E . qRT-PCR was used to detect miR-335-5p expression in normal hepatic epithelial cell line (THLE-3) and HCC cell lines (Huh7, Hep3B, HCCLM3, and MHCC97-L). F . The correlation between circ_0064288 expression and miR-335-5p expression in HCC tissues was evaluated by Pearson’s correlation coefficient. *** P < 0.001, ns, means no statistical significance. *** P < 0.001, ns, means no statistical significance

Article Snippet: Human HCC cell lines (Huh7, Hep3B, HCCLM3, and MHCC97-L) and human normal liver epithelial cell line (THLE-3) were procured from American Type Culture Collection (ATCC, Manassas, VA, USA) and Shanghai Cell Bank of Chinese Academy of Sciences (Shanghai, China).

Techniques: Binding Assay, Quantitative RT-PCR, Expressing, Luciferase, Sequencing, Functional Assay